Composite

Part:BBa_K1878004:Design

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


fdhf/iRFP670


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 186
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 741
    Illegal BsaI.rc site found at 298


Design Notes

The design of this vector lacks a ribosome binding site.


Source

iRFP670 is a mutated form of the Rhodopseudomonas palustris bacterial phytochrome photoreceptor. The fdhf promoter was copied from the E. coli genome.


References